Commencing at 6:00pm on Friday night, the match will double as former Magpies coach Ken McGregor's return to Alberton - now as the head of the Roosters.
See every Magpies home game at Alberton this year with a Magpies home game membership.
Garry Hocking's Magpies have been undefeated, albeit in trial games, throughout the pre-season campaign.
He called on Port Adelaide supporters to get to Alberton for a rare night football opportunity.
"We don't get to play night footy at Alberton in season and you can't get into many night games for free, so it would be good to have as many supporters as we can out to see us in action," Hocking told portadelaidefc.com.au.
"We play Norwood on the following Thursday under lights at the Parade, so we'll be using this as our last proper hit out before the season starts."
Boost your Power membership and see every Magpies home game at Alberton with a We Are Port Adelaide upgrade. Call the club on 1300 GO PAFC (1300 467 232) today.
Action at Alberton commences at 6:00pm, but there will be no curtain-raiser with the club's Academy side kicking off its 2014 reserves season campaign against the Roosters at Alberton on Sunday afternoon.
The Port Club's dining and Alberton Oval kiosk facilities will be open on Friday night.
Port Adelaide SANFL trial game details
Port Adelaide v North Adelaide
Friday 28 March, 6:00pm
Alberton Oval, Brougham Place, Alberton
Enter via Queen Street and Brougham Place
