Proceeds raised will go towards the club's Community Youth Program, which works with young people in schools and other community organisations to promote healthy living messages.
And it is expected that injured players Sam Colquhoun, Jarrad Redden and Campbell Heath will eventually use the bike as part of their recovery.
Port Adelaide's resident tri-athlete, current physiotherapist and former player Michael Wilson says the Trek Madone will both help the club on and off the field.
"It's really good of Bicycle Express to donate this Trek for the boys to use as part of their recovery," Wilson told portadelaidefc.com.au.
"The bike was custom painted in the USA, so the boys will feel at have a black, white and teal Madone to help in their recovery.
"Bicycle Express are also really supportive of the club's community programs and we've arranged to auction the bike off at the end of the season and the proceeds will go to the Communtiy Youth Program run by Russell Ebert.
"It's a nice little message because we all know cycling is a great way to keep fit, and the Community Youth Program is all about promoting healthy living."
Stay tuned to portadelaidefc.com.au as we cover the rehab of Sam Colquhoun over the next 12 months.